Create a Data Acquisition Log template for multiplexed qPCR instrument runs that captures well-by-well Ct values across a 384-well plate layout, thermocycler serial numbers, and standard curve metrics (R² >0.98) for gene-expression validation studies supporting IND submissions

Generate create a data acquisition log template for multiplexed qpcr instrument runs that captures well-by-well ct values across a 384-well plate layout, thermocycler serial numbers, and standard curve metrics (r² >0.98) for gene-expression validation studies supporting ind submissions for Scientific Research and Development Services industry

Scientific Research and Development Services

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384-Well Plate Layout File *

Click to upload or drag and drop

Allowed: RDML, CSV, XLSX

Max size: 50MB

Upload the qPCR instrument output file containing Ct values for all 384 wells in standard RDML or CSV format

Thermocycler Serial Numbers *

Enter the serial numbers of all thermocyclers used in this multiplexed qPCR run for equipment traceability

Standard Curve Validation Metrics *

Report R² values, slope, and y-intercept for each gene target's standard curve meeting IND submission requirements

Gene Expression Targets *

Select the gene expression targets being validated in this IND-supporting study

Study Phase Context *

Specify the IND submission phase and regulatory context requiring this validation data

Quality Control Acceptance Criteria *

Define the acceptance criteria for NTC controls, amplification efficiency, and run validation metrics

Reference Genes and Controls *

List the reference genes, positive controls, and negative controls used for data normalization and validation

Sample Preparation Protocol *

Specify the RNA extraction and reverse transcription protocol used to ensure reproducibility across studies

IND Submission Timeline *

Indicate the urgency and timeline constraints for this genetic validation data package

Additional Regulatory Context

Provide any specific sponsor requirements, previous FDA interactions, or IND-specific guidelines that must be followed for this gene expression validation

Create a Data Acquisition Log template for multiplexed qPCR instrument runs that captures well-by-well Ct values across a 384-well plate layout, thermocycler serial numbers, and standard curve metrics (R² >0.98) for gene-expression validation studies supporting IND submissions

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